• Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples 

      Jacobsen, Carsten Suhr; Nielsen, Tue Kjærgaard; Vester, Jan Kjølhede; Stougaard, Peter; Nielsen, Jeppe Lund; Voriskova, Jana; Winding, Anne; Baldrian, Petr; Liu, Binbin; Frostegård, Åsa; Pedersen, Dorthe; Tveit, Alexander Tøsdal; Svenning, Mette Marianne; Tebbe, Christoph C.; Øvreås, Lise; Jakobsen, Pia Bach; Blazewicz, Steven J.; Hubablek, Valerie; Bertilsson, Stefan; Hansen, Lars Hestbjerg; Cary, S. Craig; Holben, William E.; Ekelund, Flemming; Bælum, Jacob (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-04-09)
      Here we show that a commercial blocking reagent (G2) based on modified eukaryotic DNA significantly improved DNA extraction efficiency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the ...
    • Lake Sedimentary DNA Research on Past Terrestrial and Aquatic Biodiversity: Overview and Recommendations 

      Capo, Eric; Giguet-Covex, Charline; Rouillard, Alexandra; Nota, Kevin; Heintzman, Peter D.; Vuillemin, Aurèle; Ariztegui, Daniel; Arnaud, Fabien; Belle, Simon; Bertilsson, Stefan; Bigler, Christian; Bindler, Richard; Brown, Antony G.; Clarke, Charlotte L.; Crump, Sarah E.; Debroas, Didier; Englund, Göran; Ficetola, Gentile Francesco; Garner, Rebecca E.; Gauthier, Joanna; Gregory-Eaves, Irene; Heinecke, Liv; Herzschuh, Ulrike; Ibrahim, Anan; Kisand, Veljo; Kjær, Kurt H.; Lammers, Youri; Littlefair, Joanne; Messager, Erwan; Monchamp, Marie-Eve; Olajos, Fredrik; Orsi, William; Pedersen, Mikkel W.; Rijal, Dilli P.; Rydberg, Johan; Spanbauer, Trisha; Stoof-Leichsenring, Kathleen R.; Taberlet, Pierre; Talas, Liisi; Thomas, Camille; Walsh, David A.; Wang, Yucheng; Willerslev, Eske; van Woerkom, Anne; Zimmermann, Heike H.; Coolen, Marco J. L.; Epp, Laura S.; Domaizon, Isabelle; Alsos, Inger G.; Parducci, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-13)
      The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field in paleoecological research. Although largely applied nowadays, knowledge gaps remain in this field and there is therefore still research to be conducted to ensure the reliability of the sedimentary DNA signal. Building on the most recent literature and seven original ...
    • Multiomics in the central Arctic Ocean for benchmarking biodiversity change 

      Mock, Thomas; Boulton, William; Balmonte, John-Paul; Barry, Kevin; Bertilsson, Stefan; Bowman, Jeff; Buck, Moritz; Bratbak, Gunnar; Chamberlain, Emelia J.; Cunliffe, Michael; Creamean, Jessie; Ebenhöh, Oliver; Eggers, Sarah Lena; Fong, Allison A.; Gardner, Jessie; Gradinger, Rolf Rudolf; Granskog, Mats A.; Havermans, Charlotte; Hill, Thomas; Hoppe, Clara J.M.; Korte, Kerstin; Larsen, Aud; Müller, Oliver; Nicolaus, Anja; Oldenburg, Ellen; Popa, Ovidiu; Rogge, Swantje; Schäfer, Hendrik; Shoemaker, Katyanne; Snoeijs-Leijonmalm, Pauline; Torstensson, Anders; Valentin, Klaus; Vader, Anna; Barry, Kerrie; Chen, I-M A; Clum, Alicia; Copeland, Alex; Daum, Chris; Eloe-Fadrosh, Emiley; Foster, Brian; Foster, Bryce; Grigoriev, Igor V.; Huntemann, Marcel; Ivanova, Natalia; Kuo, Alan; Kyrpides, Nikos C.; Mukherjee, Supratim; Palaniappan, Krishnaveni; Reddy, T.B.K.; Salamov, Asaf; Roux, Simon; Varghese, Neha; Woyke, Tanja; Wu, Dongying; Leggett, Richard M.; Moulton, Vincent; Metfies, Katja (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-10-17)
      Multiomics approaches need to be applied in the central Arctic Ocean to benchmark biodiversity change and to identify novel species and their genes. As part of MOSAiC, EcoOmics will therefore be essential for conservation and sustainable bioprospecting in one of the least explored ecosystems on Earth.